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KMID : 1059519910350030219
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Journal of the Korean Chemical Society
1991 Volume.35 No. 3 p.219 ~ p.225
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Application of Home-made Capillary Zone Electrophoresis System to the Separation of Organic Molecules
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Lee Kong-Joo
Heo Gwi-Suk
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Abstract
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Capillary zone electrophoresis (CZE) which is highly efficient separation technique has been domestically established having optimum detection sensitivity. By applying 20∼35 kV of electric potential to the narrow (50 μm i.d.) capillary tubing filled with running buffer, this technique can quickly (< 20 min) separate the small quantities of sample with high separation efficiency (number of theoretical plates : 200,000∼500,000). Factors affecting the separation efficiency and resolution in CZE were examined by analyzing adenine and catecholamine derivatives.
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KEYWORD
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